![]() ![]() This BioBrick encodes for the Envirowire fusion protein. This image was created using SnapGene Viewer.) Illustrates the construction of BBa_K1739003. Envirowire: Sup35NM with an N-terminal CsgAss signal sequence and a C-terminal Cytochrome b 562 This part has been inserted into the pSB1C3 backbone and uses the promoter BBa_J23104. The particular advantage of using Sup35NM is that in its native state Sup35p has two functional domains, the N and C terminal, separated by the highly charged M domain allowing the fusion of a new functional domain. This has previously been discussed by Tessier and Lindquist (2009) who show that two beta-sheets bond together in a self-complimenting ‘steric zipper’ that excludes water, leaving a highly stable parallel beta-sheet with one molecule every 4.7 Angstroms. cerevisiae (bakers yeast) and excludes the C-terminal domain with the N-terminal domain allowing self-assembly of functional amyloid. Sup35NM is derived from the yeast prion protein Sup35p from S. This part design includes the bipartite CsgA signal sequence that targets the protein to the Sec-export pathway and subsequently to the Curli export pathway via interaction with CsgG. The image was created using SnapGene Viewer.) Illustrates the construction of BBa_K1739002. Sup35NM with N-terminal CsgAss signal sequence Cytochrome b 562 is a single subunit, four-helix bundle protein containing a non-covalently bound b-type haem group with a molecular weight of 25kDa. ![]() This part encodes Cytochrome b 562 in a pSB1C3 backbone. Illustrates the construction of BBa_K1739001. ![]()
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